Composite

Part:BBa_K4171014:Design

Designed by: HUANG, YEN-JUNG   Group: iGEM22_NCKU_Tainan   (2022-08-16)


PT7-tRNA(Ser)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 58
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

None


Source

1. T7 promoter sequence derives from the T7 bacteriophage

2. tRNAser sequence derives from Escherichia coli

References

Asahara, H., Himeno, H., Tamura, K., Nameki, N., Hasegawa, T., & Shimizu, M. (1994). Escherichia coli SERYL-trna synthetase recognizes tRNASer by its characteristics tertiary structure. Journal of Molecular Biology, 236(3), 738–748. https://parts.igem.org/Part:BBa_I719005